Clara V Alvarez

2 Questions 5 Answers 0 Followers

Questions related from Clara V Alvarez

Which is the minimal transfection efficiency that we should accept for transient transfection?

Recently, a call for improving basic research protocols in order to get results more relevant for the clinical setting has been made. Transient transfection is a wide used technique in current...

01 January 2015 8,390 3 View

Is it possible to delete a gene from a cultured cell with the new directed DNAses system (CRISPR, /CASP, TALEN, ZFN)?

I have human epithelial primary cultures. I would like to delete specific genes to reproduce certain diseases where gene mutations equals to absence of protein. Is it possible to do it with these...

02 February 2014 1,574 4 View