Claire Thornton

4 Questions 28 Answers 0 Followers

Questions related from Claire Thornton

Identifying a large molecular weight complex by size exclusion chromatography - would prior low power sonication disrupt complex?

We'd like to identify the components of a complex of proteins from a protein lysate. If we lyse the cells under non-denaturing conditions (i.e. osmotically) the complex should weigh in around...

01 January 2014 2,172 14 View

A quick protocol for extracting DNA from human saliva?

Not averse to using a kit but if anyone has a quick protocol to generate DNA that can be used for qPCR etc..?

03 March 2013 4,603 14 View

Neurospheres attaching after passage.

Growing mouse cortical/hippocampal neurospheres in DMEM:F12/B27/glutamine +FGF +EGF, and initially lots of free-floating neurospheres. After passage, neurospheres attaching to the (uncoated) 6...

03 March 2013 4,701 7 View

Western blot antibody for Quiescence?

I'm working on apoptosis but recently found that instead of dying, my C17.2 cells were going into quiescence, (I think it is anyway, as it is reversible once the insult is removed). Just want to...

02 February 2013 6,304 16 View