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Questions related from Claire Louise Morley
I am genotyping ear snips from GMO mice, the parents are both hets to make a mixed litter of WT (+/+), het (+/-) and KO (-/-) for the target gene. I have a pair of WT primers and a pair of KO...
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I want to use ct values as an indicator to the presence or absence of the target. The samples without reverse transcriptase contained no DNA. But my target samples had a high ct value (>35). Can I...
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