Claire Gates

3 Questions 2 Answers 0 Followers

Questions related from Claire Gates

Do HCT-8 colon cancer cells require horse serum?

Hi all, I am thawing some HCT-8 cells from ATCC and it calls for RPMI with horse serum. Does anyone know the reasoning for this and if they will tolerate FBS? Thanks, Claire

06 June 2022 5,902 1 View

Has anyone ever had challenges with doing site directed mutagenesis with a previously working primer?

Hi all! I am doing site-directed mutagenesis to implement SNPs in a vector. I have successfully gotten all my single mutations and am now moving into creating haplotypes, so for example I'll take...

17 January 2020 9,168 4 View

Does anyone have any advice for troubleshooting recombination step using the bac-to-bac baculovirus expression kit?

Hi all, Hope you are having a nice day! I was wondering if anyone has used the bac-to-bac baculovirus expression kit to express recombinant protein? There is a step when creating the virus, where...

08 October 2019 8,779 1 View