RNA seq: How many reads (5, 10, 30M) do I need to perform differential expression analysis?

I am planning to perform RNA seq using a MiSeq Reagent Kit v3 600 cycle, mean insert size of ~600bp, 2x 300bp reads, paired-end. I have had some divergent advice regarding the no. of reads...

19 July 2016 1,472 9 View