How to get transformed E. coli strains using Promega´s pGL3 Luciferase expression vector containing human promoter? Does anyone had the same problem?

I respectively cloned two different promoters from human genes on Promega´s pGL3 vector but I can't obtain any E. coli transformants. The transformation with pGL3 basic control vector works pretty...

02 June 2016 1,667 3 View