Cihan Inan

5 Questions 6 Answers 0 Followers

Questions related from Cihan Inan

CRISPR-Cas9 expression vector suggestions for insect cell line ?

I am planning to edit a viral (insect virus) genome using CRISPR CAS-9 system. I need to find a suitable vector system for producing cas-9 and gRNA's in insect cell culture. Any suggestions?

28 November 2017 9,767 1 View

Detecting homologous regions (hrs) in Lymantria dispar nucleo polyhedrovirus ( LdMNPV )

I would like to learn is there any software for detecting Lymantria dispar nucleopolyhedrovirus hrs regions? I am working on a full genome sequence data and I need to search hrs regions in my...

06 July 2017 3,904 2 View

Is it possible to stop transcription before stop codon and how?

I sequenced 3' RACE products and my stop codon is missing. Is it possible to terminate transcription before stop codon?

15 July 2014 8,906 2 View

Is there any relationship between transmembrane domains and 3' UTR regions?

I am using Roche 3'/5' 2nd generation RACE kit to determine 3' and 5' utr regions. I have completed all steps and sequenced my 3' plasmids (pGEM-T Easy vector). The main point: my gene has a...

10 June 2014 2,592 1 View

I need Vaccinia virus DNA for my project.

Hi all; I am working on entomopoxviruses and I need vaccinia virus DNA for my experiments. I created a recombinant virus lacking a gene in AMEV genome (Type member of betaentomopoxviruses) and now...

01 January 1970 4,754 1 View