Çiğdem Yılmaz

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Questions related from Çiğdem Yılmaz

Are primers used in reverse transcriptase PCR also used in real time PCR?

Are primers used in reverse transcriptase PCR also used in real time PCR? Or should I redesign the appropriate primers? If so, what is the difference?

12 December 2019 1,828 4 View

I cannot detect Parp protein after total protein isolation?

I cannot detect Parp protein after total protein isolation Hello everyone; I cannot detect the Parp protein after total protein isolation. I can see my cells die morphologically after a novel...

07 July 2019 9,691 2 View

I can't detect cleaved caspase-3 with western blot?

Hello to everyone. I cannot detect cleaved caspase-3 with western blot in the cells I 48 hours treat with anti-cancer agent. Annexin V and cParp were confirmed by apoptosis. My transfer...

03 March 2019 5,757 6 View

DNA, RNA and protein. What is the ranking of their molecular size?

For example, when RNA is isolated from a cell, how do these molecules (DNA,RNA,protein) pass through the pores?

01 January 2019 1,593 0 View

What do you think about the state of the cell?

HEK-293T is becoming this for a while after being in culture. (20X magnification) Other cells in the culture have no such problem. Can you describe this morphology?

01 January 1970 9,951 3 View

Is GC content important when designing oligonucleotides for transfection?

Is GC content important when designing oligonucleotides for transfection? Is this a problem for oligonucleotide-based transfection, where the dna region I will design oligonucleotide is very rich...

01 January 1970 2,448 3 View