Ciara Christianne Lim

2 Questions 2 Answers 0 Followers

Questions related from Ciara Christianne Lim

What if the elution buffer for protein purification turns yellow?

I prepared a buffer containing the following: 50mM sodium phosphate, 250mM imidazole, 3M NaCl and 50% glycerol. When I autoclaved them all together, the solution turned yellowish. Is it still okay...

08 August 2013 4,748 6 View

What is the best high-fidelity polymerase to use for subsequent sequencing and protein expression?

I'm thinking between Invitrogen's Platinum Taq Hotstart, Kapa Biosystem's HI-FI Hotstart and NEB's Phusion high-fidelity. I'm not sure which will yield the best amount of product and specificity,...

03 March 2013 3,267 7 View