Chuxuan Hu

2 Questions 2 Answers 0 Followers

Questions related from Chuxuan Hu

How to extract membrane protein from the primary immune cell for the further IP steps?

Hi all, I am trying to identify a receptor in primary immune cells for my protein of interest, but I am not seeing any potential bands in the elution buffer. For the immunoprecipitation (IP), I...

08 July 2025 7,835 3 View

Questions about Primary NK cells in Transwell migration assay?

Hello all, I am currently performing a transwell assay with primary NK cells. I have tested this system under several conditions, including the addition of HIFBS, varying incubation times (1 to 2...

12 December 2024 1,422 1 View