Chutima Chanprasert

2 Questions 3 Answers 0 Followers

Questions related from Chutima Chanprasert

Has anyone used Lipofectamine LTX + Plus Reagent for transfection in HT-1080cells?

I performed transfection in HT1080 with Lipofectamine 2000. i got only 20-30% transfection efficiency.

17 December 2024 8,175 0 View

Why am I not getting expected pCDNA3.1 plasmid containing large insert after digestion?

I have cloned a 6.46 kb having restriction sites for enzymes BamHI and Not I in pcDNA3.1 vector. After colony pcr screening, the result shows positive result with primer specific to vector and...

16 May 2024 199 4 View