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We are trying to stain cholangiocytes with mitotracker green and perform confocal live imaging. However, once we added mitotracker, the entire monolayer of cells started to peal off from the...
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I am looking for antibody that can mark mitochondria on paraffin sections. Protocol will also be highly appreciated. Thank you in advance!
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I am looking for ways to do fluorescent labeling for senescence cells in zebrafish. Thank you very much.
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I would like to make fusion constructs to tag protein in different cellular compartments (ER, Golgi, endosomes). Need recommendation on what tags to use.
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I PCR amplified my gene of interest, both the WT form and a mutant form with a missense mutation. The gene encodes an ABC transporter and is about 4kb. Both PCR products looked good on the gel and...
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I have never done IP before and need some advice on the experiment planning: I will collect cell lysate and incubate it with an antibody of my interest. The antibody is rabbit polyclonal IgG. I...
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I am trying to isolate livers from 5 day old zebrafish for western, but can't get enough protein. I already maximize the no. of fish I can humanly dissect. Is there a way to do western on very...
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