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hi, I have used FV3000(Olympus) to scan the mouse brain slices and I am trying to find the best step size of Z section to get a good quality of image as well as save time. I tried to used the...
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Hi ALL, I am trying to do the qPCR and detect the BNDF gene in the mammary tissue. But the CT in my experiment is about 32. I know it means nothing! But in our previous experiment, the CT is...
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I did a qPCR of mammary tissue with two housekeeping genes (GAPDH and B-actin), but the Ct value is unstable among the control and the samples. Most of the Ct are around 21, but some of them are...
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I am working with the RNA isolation of mammary tissue. I have used TRIzol (500ul) and chloroform(200ul) to isolate the RNA. But after spin at 12,000g for 15min, I got a pink layer at the bottom...
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