I use 0.05% EDTA-trypsin to detach Hep G2 cells, and I culture them in DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA),but my cell will clump?

i use 0.05% EDTA-trypsin to detach Hep G2 cells 6min then neutralize the trypsin- EDTA with DMEM(HG) with 10% FBS, 1% p/s, and 1(NEAA) and centrifuge gently then resuspend cells in fresh...

11 December 2023 349 5 View