Chun Hin Law

5 Questions 8 Answers 0 Followers

Questions related from Chun Hin Law

How should I treat my iTRAQ data when I have 2 biological replicates and 3 technical replicates?

In my experiment, I have 2 iTRAQ samples and each has been run 3 times. I would like to know how I should report these data? average them all? or I should treat them differently. Also, what should...

02 February 2013 6,423 4 View

My triple quadrupole got corroded. Is there anyway to get it back? Or would it affect my signal a lot?

I saw some brass color and rainbow pattern on my stainless steel quadrupole when I cleaned it. I previously done some analysis of lipids and pesticide with acidic solvent (1% acetic acid in...

01 January 2013 8,886 8 View

How can I separate my E coli from my cell culture?

I used E coli to infect my cell (J774 macrophage) and want examine the proteome of J774 after infection by shotgun proteomics. Though I can filter the spectra corresponding for the E Coli using...

12 December 2012 1,811 7 View

I am using a AB Sciex 5600 and I want to do spectral count for quantitation. Which software would you suggest? Thanks.

Actually, I'm using a AB Sciex 5600 and ProteinPilot for database search. I want to do spectral count quantitation afterwards. Are there any free software available for the purpose? Thanks.

10 October 2012 5,812 4 View

How to purify proteins from RIPA lysis buffer without using acetone precipitation?

I am looking for a protocol to purify proteins from RIPA. I need to do iTRAQ afterwards, so excess urea is not a good idea. Please let me know if you have any suggestions. Thanks.

09 September 2012 1,491 3 View