Chu-Wei Huang

2 Questions 2 Answers 0 Followers

Questions related from Chu-Wei Huang

Lentivirus production problem?

Hello I'm producing lentivirus using HEK293FT cells. The plasmid ratioI used was 4:3:1 with expression plasmid(pSIN backbone):p8.91:pMD.G.I used calcium phosphate to transfect 293FT cells (10cm...

27 May 2016 154 2 View

Can primary cells isolated from embryonic stage express Cre recombinase which should be expressed in postnatal stage?

I've generated a conditional KO mice which could express CRE recombinase in the forebrain after postnatal day 3. Can anyone tell me that whether the primary neuron could still express Cre if I use...

17 November 2014 5,086 3 View