Christy Eslinger

5 Questions 1 Answers 0 Followers

Questions related from Christy Eslinger

Has anyone dealt with their bait protein falling behind the heavy chain when attempting a RIME assay?

I am currently trying to pull down my bait protein that comes at the 55 KD range. My first attempt at the RIME did result in hundreds of hits but my bait protein was not seen. I believe it is...

22 October 2021 8,629 0 View

Looking for an elution protocol, using urea, for protein of interest from Protein A Sepharose 4B conjugate beads?

I performed an IP protein experiment where I pulled down the protein of interest and attached it to a protein A sepharose 4B bead. I am planning to send this for mass spec at our core facility....

19 November 2020 716 1 View

Looking for a better RNA extraction protocol from HEK cells?

This is a time study that I am performing where the cells are exposed to TNBS in SFM at different time points. I'm then waiting 24 hours in normal media and then harvesting the cells for DNA and...

26 June 2020 8,563 0 View

Preference question: ChIP Assay vs Native ChIP?

Has anyone had better luck using a Native ChIP rather than the traditional ChIP Assay? I've been hearing that it is a more accurate analysis than the ChIP. Any opinions?

08 February 2020 5,132 0 View

Best way to dissolve protien pellet following TCA in acetone w/DTT and washed with acetone an DTT?

Extracting total protein from plant leaf and used a protocol where the lysis buffer is 10%TCA in acetone w/ 0.007g DTT added. The pellet was then re-suspended in acetone and DTT. This also was the...

12 January 2020 6,330 3 View