Christopher Willig

3 Questions 5 Answers 0 Followers

Questions related from Christopher Willig

What publicly available dataset(s) can I use as a proxy for estimating what proportion of a given genome is functionally characterized?

I want to be able, in some quantitative way, to compare several plant species with respect to how much is known about the functions of all known loci in their genomes. It seems like looking at...

10 October 2018 2,630 4 View

How can I perform a blunt-end ligation with a large vector and large insert?

I am trying to perform a rather difficult blunt-end ligation that requires several enzymatic steps and would appreciate any tips from those who have experience with blunt-end cloning. My...

06 June 2018 5,050 11 View

Should I digest with BsaI and gel purify my destination vector prior to setting up a Golden Gate reaction for cloning gRNA spacer sequences?

I'm planning to use Golden Gate cloning in order to insert guide RNA spacer sequences into a cloning vector between two head-to-head BsaI restriction sites. Prior to setting up the reaction with...

03 March 2018 7,911 1 View