Christopher Gaughan

3 Questions 6 Answers 0 Followers

Questions related from Christopher Gaughan

Crosslinked proteins run in SDS_PAGE gel as smear - any thoughts?

I have crosslinked two proteins using typical crosslinking reagents. However, the crosslinked product runs as a smear instead of a tight band. Does anyone have an explanation for this? Does anyone...

06 June 2014 4,367 4 View

Has anyone ever found that PMSF can affect enzyme activity of recombinantly expressed proteins?

I am expressing 2 enzymes in BL21, one is the WT form and the other a variant. I expressed the WT form without using any PMSF in the lysis buffer and the activity is fine. However, when I express...

08 August 2013 7,634 13 View

Question about enzyme kinetics

I am performing a reaction in which the [S]>>[E]- I'm obtaining and v vs [E] curve. In Michaelis Menten, one plots 1/[S] vs 1/[v] to get enzyme parameters (Km, etc). Can I plot 1/[E] vs 1/v and...

03 March 2013 9,025 1 View