Christopher Mayhugh

4 Questions 1 Answers 0 Followers

Questions related from Christopher Mayhugh

What method/equation is most reliable for calculating ELISA cut-off value?

Hi, I performed an animal immunization study for the first time in a mouse model, and have serum and bronchoalveolar fluid for indirect ELISA analysis. As a result, I do not have a standard curve...

29 March 2023 7,260 3 View

What other methods exist for removal E. coli endotoxin from recombinant proteins?

I'm a PhD student working on protein engineering & immunology and making recombinant proteins in lab for work with mammalian systems. I have tried using ClearColi previously (BL21 DE3 w/...

22 March 2023 5,719 3 View

How long can I keep an antigen-coated ELISA plate at 4C?

Planning to run my assays this week, but not sure if I will be able to tomorrow. I want to prep as much as possible ahead of time, and was wondering if I could coat my plates with 10ug/mL antigen...

12 March 2023 9,142 6 View

How do you calculate Ab titer from indirect ELISA?

Hi, My lab is beginning to delve into immunological assays, and I am going to run some indirect ELISAs using serum IgG collected from mice in an immunization study. I'm a bit confused on how to...

19 April 2022 9,962 4 View