Could anyone tell me why the protein contained in my lysate is precipitated when DNAse is added? I'm losing most of my proteins in this step.

I'm trying to isolate proteins to perform an IEF of Leishmanial proteins, so I have to eliminate all the DNA present in the lysate. The problem is that 10 min after the incubation with DNAse (20...

13 November 2012 6,509 18 View