Christopher M. Bartley

3 Questions 8 Answers 0 Followers

Questions related from Christopher M. Bartley

Which produces lower background on phage display (less non-specific binding), agarose or magnetic beads?

I'm curious whether anyone has performed parallel phage display assays using magnetic and agarose beads to compare nonspecific binding. Any sense for which produces lower background binding?

28 March 2018 9,758 2 View

Is it possible to knock a transgene into the mitochondrial genome?

I'm interested in expressing a synthetic gene from the mitochondrial genome. It is possible to knock a transgene into the mitochondrial genome such that it's passed on to subsequent generations?

28 June 2016 5,800 3 View

Does crosslinking an antibody to protein A or G beads alter the efficiency of immunoprecipitation?

Assuming all other conditions are equal, any idea what the relative efficiency of immunoprecipitating a protein using noncovalently bound antibody vs covalently crosslinked antibody is?

11 April 2015 7,616 9 View