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Questions related from Christoph Matti
We are experiencing a decrease in protein expression in our BL21 DE3 strain. So far we were able to rule out an influence of i.e. IPTG (prepared new fresh stock), however we have no clue what else...
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Dear all, we are trying to change our lysis method for membrane receptors (using Molon buffer, 15min ice, 15min centrifugation 14'000g, then overnight on beads) with receptor-HA constructs in...
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Dear all, I want to purify a final tagging reaction, where a 12kDa protein gets a chemical 1.5kDa tag via enzymatic method by a 60kDa transferase. As it is quite sticky, I don't want to use FPLC...
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Dear all, I performed several qPCR, which also worked fine, however, now during the evaluation I encountered an issue. My problem is that due to the restriction when choosing primers, I ended up...
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I am developping an ELISA, which seems to work, although I am puzzled by the titration of the capture AB. The Assay: All samples were in dublicates, volume used 50ul, except 150 for blocking,...
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