Christof Gaunt

2 Questions 6 Answers 0 Followers

Questions related from Christof Gaunt

Optimal PBMC freeze/thawing conditions for monocyte viability and functional preservation

Hi all, I am looking for a protocol detailing the optimal freezing and thawing conditions of PBMCs to ensure maximum viability and functionality of monocytes. I will be freezing down patient PBMCs...

07 July 2017 9,017 1 View

Does anyone have a database of both fluorochromes and cell antigens that are sensitive/resistant to methanol permeabilisation?

I am staining for intracellular pSTAT1 and I am repeatedly running into difficulty owing to the fact I have to permeabilise with methanol (for sufficient nuclear entry of anti-pSTAT1 antibody and...

09 September 2016 2,263 4 View