Christine Yougn

4 Questions 3 Answers 0 Followers

Questions related from Christine Yougn

Can anyone recommend the best way to thaw cryopreserved spleen cells?

We have frozen (10% DMSO, 90% FBS) spleen cells which we want to thaw and enrich for CD8+ cells. Can anyone recommend the best way to thaw theses cells - would you use dnase treatment, and what...

27 July 2019 5,522 2 View

How long to leave CD8+ t cells in culture before the are stimulated and expand for a T cell killing assay?

I am trying to stimulate and expand t cells. I am using dynbeads to perform a negative selection for CD8+ cells from mouse spleen. after selecting CD8+ cells, I start of with around 1 million...

10 July 2019 1,202 11 View

Old Qiagen RNeasy On-Column DNase vs new gDNA Eliminator columns - any thoughts?

Has anyone tested the efficacy of the new Qiagen gDNA Eliminator columns at removing genomic DNA from RNA preps (as part of the Qiagen RNeasy Plus kits)? Are they better than their old On-Column...

22 October 2018 5,046 4 View

Old Qiagen RNeasy On-Column DNase vs new gDNA Eliminator columns - any thoughts?

Has anyone tested the efficacy of the new Qiagen gDNA Eliminator columns at removing genomic DNA from RNA preps (as part of the Qiagen RNeasy Plus kits)? Are they better than their old On-Column...

10 October 2018 2,512 1 View