Christine A. Ikpeme

2 Questions 6 Answers 0 Followers

Questions related from Christine A. Ikpeme

Besides the issues of buffer pH in relation to pI, what other factors could make an enzyme protein not to bind to a cation or anion exchange resin?

The cellulase enzymes I am purifying did not bind to DEAE cellulose and CM sapharose columns using an acetate buffer, pH 5.0. The pI of the enzyme protein is 7.0. Please could someone help me with...

01 January 2020 4,548 13 View

Please i need an expert help on how to convert amino acids results expressed mg/l to residues??

Please what formula should I use to convert amino acid compsition results expressed in mg/l to residues? I ran a HPLC amino acid profile of a protein sample, the results came in mg/l but I need to...

01 January 2020 3,963 1 View