Christine Dwi A P W

5 Questions 6 Answers 0 Followers

Questions related from Christine Dwi A P W

Is it possible to construct a PCR primer based on the EST Clone data?

From the Microarray data (based on the EST clone), I found several genes that needs deeper research to find out the gene expression under heavy metal stress. Thus, we need to conduct an qPCR to...

07 July 2016 1,788 3 View

Can anyone suggest some DNA plasmid isolation protocols?

I'm going to isolate plasmid from unknown soil bacteria, is there any simple plasmid isolation protocols? Since using a kit is actually my last option, so I'm trying to use manual protocols...

09 September 2014 1,159 2 View

Can you give some examples of efflorescence and deliquescent compound?

From what i know, here's a list about some efflorescence and deliquescent compound: Efflorescence: CoCl2, Na2CO3, Na2SO4 Deliquescent: CaCl2, MgCl2, ZnCl2 Then what about the other hydrated...

09 September 2014 8,936 3 View

Do all of Mer genes (in all of bacteria) have some kind of similar sequence?

Is there any similarity between Mer genes sequence in all bacteria? i'm trying to isolate mer genes from unknown bacteria from gold mining site in Yogyakarta, Indonesia. If all of the mer genes...

08 August 2014 1,546 2 View

Why are the 260/280 ratios higher than 2 after DNA purification?

I'm doing DNA extraction using Chelex and before DNA purification, it have 260/280 ratio start from 1,1-1,4. Usually after DNA purification, 260/280 ratio will ranging between 1,8-2 (Pure DNA) but...

08 August 2014 7,685 21 View