Christine Ebeid

3 Questions 10 Answers 0 Followers

Questions related from Christine Ebeid

Can prokaryotic methylation of plasmid DNA during cloning affect this plasmid after being isolated and transfected in eukaryotic cells?

Upon cloning of plasmid DNA in Chemical competent E-coli cells (having dcm and dam), followed by its isolation using mini prep and using this plasmid for transfection in 3T3 cells ( murine...

16 September 2016 3,331 3 View

The reason why dual luciferase assay showed low signal of experimental vector compared to the control vector?

I'm trying to detect promoter activity using Dual luciferase assay. Several promoter truncated fragments were PCR amplified, their sizes ranged from 200 bp to 5000 bp, followed by their...

08 September 2016 575 20 View

Why I don't get positive clones (having my insert)?

I'm trying to clone a 4 Kb insert into PNl1.1 vector (3 Kb). I'm digesting both the vector and the insert with KpnI & XhoI and using alkaline phosphatase for the vector only. Also, I'm doing...

17 May 2016 1,781 3 View