FACS analysis: Why would cells treated with a high drug dosage be 7-AAD positive but Hoechst negative? Considering both dyes intercalate with DNA?

I have been dual staining cells treated with drugs and at lower doses I get the characteristic shift to Hoechst stained and then dual stained with hoechst and 7-AAD stained. However for a higher...

11 November 2015 7,110 5 View