Christina Desnoyers

4 Questions 7 Answers 0 Followers

Questions related from Christina Desnoyers

I'm having an issue with an exogenous internal positive control; is the absolute magnitude of fluorescence important (qPCR end point RFU values)?

I would like to use an exogenous internal positive control in my qPCR reactions (probe-based with gDNA). The IPC protocol states a 60C anneal/extend step, but my target assays require this step to...

07 June 2021 5,236 5 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

19 January 2021 2,096 6 View

Why is there a streak starting from the well and then fading out on gel?

Gel in the attached image: 1% agarose, 100 V, 50 minutes, 100 bp ladder, 6ul was loaded in each well (1ul of 6X loading dye and 5 ul DNA). I was expecting to not see any bands in this gel, as I'm...

20 August 2019 4,740 4 View

Can I use UltraPure sterile water to dilute DNA?

Is it OK to dilute a DNA extract with UltraPure sterile water? I was trying to measure sample concentration with a Qubit 3.0 Fluorometer , but the readings were too high and I needed to dilute my...

20 June 2019 2,906 4 View