Christiane Schnabel

3 Questions 8 Answers 0 Followers

Questions related from Christiane Schnabel

Why does fluorescent WB detection fail while chemiluminescence is fine?

I have a simple western blot of CD31 in HUVEC lysate. With an anti-mouse-HRP secondary Ab I get a good signal/band. With fluorescent secondary antibody there is no signal. I have tried multiple...

08 August 2019 5,515 4 View

Toluidine blue staining for cryo sections?

Hi! I want to stain cryo sections of artherosclerosis lesions for metachromatic cells using Toluidine blue. It seems fixation and pH condition are crucial for this staining. Can anyone provide a...

06 June 2018 4,138 3 View

Can anyone provide advice for the purification of His-tagged protein from ExpiCHO supernatant?

From transient e get very high levels of secreted recombinant protein with a N-terminal his-tag, but our purification results are poor (less than 5% yield). We have tried desalting the...

01 January 2017 6,095 7 View