Dear Experts, please why did cellulase I extracted from malted sorghum not bind to either DEAE Cellulose or CM Sepharose during ion exchange?

I am running a purification of cellulase from malted sorghum. After ammonium sulphate precipitation, a pH and buffer stability test showed the enzyme was more stable in acetate or citrate buffers...

12 December 2019 2,752 2 View