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My lab wish to establish a protocol for immuno EM of mouse sciatic nerve. Does anyone a an established protocol, or can refer to labs/papers doing immuno EM on sciatic? So far, we encounter...
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Cells need to be isolated from tissue for cell specific transcriptome analysis and it takes around 2h at 37C. Can e.g. inhibitors of RNA pol II be added to avoid new mRNA transcripts, and can...
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