Is Triton X-100 really necessary for Immunohistochemistry of fixed tissue?

For my stainings of 3 um thick slides of mouse liver tissue (fixed for 24 hrs at 4C, 4% paraformaldehyde) I accidentally didn´t use Triton X-100 to permeabilize cell membranes. I did it the same...

19 June 2016 9,869 9 View