Christian Augsberger

2 Questions 2 Answers 0 Followers

Questions related from Christian Augsberger

What is the optimal gamma irradiation dose to induce cell cycle arrest in tumor cell lines?

We are regularly irradiating mouse fibroblasts and human PBMCs with doses between 60-90 Gy. Does anyone have experience with irradiation of the myeloid cell lines HL-60, OCI-AML3, MOLM-13, KG-1a....

21 February 2018 9,956 1 View

Can you recomment a kit/protocol for the signal amplification of a FACS staining?

I am currently using a human IgG primary antibody and a Alexa647 anti-human Fc secondary antibody for the staining and do not see a signal for the low-abundance target. Thank you very much!

28 August 2017 8,364 4 View