Christiaan Kinderman

3 Questions 2 Answers 0 Followers

Questions related from Christiaan Kinderman

Plasmid isolation yields DNA, but does not show up on agarose gel?

I've isolated a plasmid with an alkaline lysis protocol, added RNAse and then measured the DNA concentration with a nanodrop. The yield was an average of 1300-1800 ng/ul. Yet, when pipetting 2 ul...

12 December 2019 2,591 10 View

Transformation of competent E. Coli not working, positive control works?

I'm attempting to perform a transformation that has succeeded the first time with different oligoduplexes and then failed 4x with different oligoduplexes, which in theory work just fine. Protocol...

12 December 2019 1,639 1 View

Check for binding of crRNA to tracrRNA?

For my internship I have to develop crispr-cas9 guides for certain genes. One of the things I have to validate in silico, is whether or not the crRNA has the ability to bind the tracRNA sequence...

09 September 2019 1,685 1 View