Chris E Buddenhagen

3 Questions 16 Answers 0 Followers

Questions related from Chris E Buddenhagen

Is GBS sequencing for phylogenetics effective on herbarium specimens?

There seems to be a requirement for high quality DNA. But if run with herbarium specimens maybe sequence capture and assembly are poorer relatively but adequate for phylogenetics with a reduced...

01 July 2018 7,447 3 View

Have you had problems with silica and lignin affecting plant DNA extraction success? How did you recognize and deal with it?

I am working in Cyperaceae and suspect that in some cases its well known high silica content (or possibly lignin) may be causing problems with getting high quality DNA during extraction using...

16 January 2014 5,811 9 View

How do you align DNA sequences for phylogenetic work? What is the order of events and what software do you use?

I anticipate having to align sequences from hundreds of loci for more than 100 taxa. What is the most efficient way to go about this? I usually use muscle (or clustal), then manual alignment in...

29 August 2013 6,355 24 View