Chottiwatt Jittprasong

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Questions related from Chottiwatt Jittprasong

I can’t PCR human TOPORS gene (Around 3000 bp) from cDNA template. Have anyone ever had success with PCR of long sequence from cDNA template?

I tried to amplify TOPORS gene of around 3,000 bp from cDNA template with no success. I use Phusion High-Fidelity DNA Polymerase to amplify the TOPORS gene. DMSO was used as well as TOPIRS is...

28 June 2023 1,883 4 View

Trying to clone human TOPORS gene into AAV vector. But encountered many anomalies. How to approach this?

Trying to clone human TOPORS gene into AAV vector plasmids. The TOPORS gene was cloned from cDNA by PCR and purified. Restriction enzyme digestion was used to isolate existing vector and make it...

26 June 2023 2,699 5 View