5 Questions 1 Answers 0 Followers
Questions related from Choco Michael Pagayatan Gorospe
I would like to measure mitochondrial mass, and wanted to use the Nonyl Acridine Orange (Acridine Orange 10-Nonyl Bromide). I have used the MitoTracker Green for measurement, although it might...
29 September 2020 4,398 4 View
Hi everyone, I hope everyone can help me with this. First, I am measuring mitochondrial membrane potential by flow cytometry using TMRE and MTG dyes. I think we are not experiencing problems with...
18 September 2020 8,854 4 View
Hi colleagues, I am trying to measure mitochondrial membrane potential and mitochondrial mass in S. cerevisiae with TMRE and mitotrackerGreen as dyes using flow cytometry. However, I always see...
28 May 2020 7,797 3 View
I usually do my blocking for 1 hour at RT using 5% skim milk, but now I am doing it already for 2 hours or so because the background is not clean with just an hour blocking. My skim milk powder is...
10 September 2015 9,876 2 View
I am making a tag for ATG8 for my western blot analysis. I was able to construct ATG7 and ATG1 tagged with TAP but not with ATG8. I was checking on some papers and noticed that all or almost all...
30 November 2014 7,334 3 View
