Choco Michael Pagayatan Gorospe

6 Questions 1 Answers 0 Followers

Questions related from Choco Michael Pagayatan Gorospe

RO-3306 for yeast cells G2 synchronization?

I was wondering if anyone has tried to G2 synchronization of yeast cells using the drug RO-3306. And if the drug works, as in mammalian cells, what concentrations have you used and treatment...

07 May 2021 3,985 0 View

Has anyone used Nonyl Acridine Orange (Acridine Orange 10-Nonyl Bromide) for mitochondrial mass measurement?

I would like to measure mitochondrial mass, and wanted to use the Nonyl Acridine Orange (Acridine Orange 10-Nonyl Bromide). I have used the MitoTracker Green for measurement, although it might...

29 September 2020 4,440 4 View

How fast is mitochondrial degradation by mitophagy in yeast?

Hi everyone, I hope everyone can help me with this. First, I am measuring mitochondrial membrane potential by flow cytometry using TMRE and MTG dyes. I think we are not experiencing problems with...

18 September 2020 8,911 4 View

How to deal with two peaks in S. cerevisiae mitochondrial mass and MMP using flow cytometry?

Hi colleagues, I am trying to measure mitochondrial membrane potential and mitochondrial mass in S. cerevisiae with TMRE and mitotrackerGreen as dyes using flow cytometry. However, I always see...

28 May 2020 7,864 3 View

Can I use higher concentration of skim milk for western blot blocking?

I usually do my blocking for 1 hour at RT using 5% skim milk, but now I am doing it already for 2 hours or so because the background is not clean with just an hour blocking. My skim milk powder is...

10 September 2015 9,909 2 View

Why is ATG8 always tagged with GFP and not with TAP?

I am making a tag for ATG8 for my western blot analysis. I was able to construct ATG7 and ATG1 tagged with TAP but not with ATG8. I was checking on some papers and noticed that all or almost all...

30 November 2014 7,377 3 View