Can you help me to establish a protocol for lysing E.coli, extracting a soluble protein, and then purifying it ?

Hello, can you help me ? I'm working with E.coli BL21 transformed with a plasmid that codes for a 13.271 kDa His-tagged periplasmic protein. I've already done the induction part with IPTG, but...

17 January 2024 112 3 View