Chloe Guest

2 Questions 6 Answers 0 Followers

Questions related from Chloe Guest

Protein attached to biotinylated antibody, which is attached to streptavidin-sepharose beads.. running on a Western?

We have a protein that we pulled out of human plasma using a biotinylated polyclonal antibody and streptavidin-sepharose bead complex. We now want to run this on a Western to see what protein we...

11 November 2018 907 1 View

ELISA Parallelism - Endogenous Compound Validation - what could cause decrease in concentration with increasing dilutions?

Four urine samples from different donors were measured on a commercial ELISA in 1:2 serial dilution from neat to 1/64. Samples were diluted in the assay buffer used to prepare the standard...

11 November 2018 1,988 9 View