Chloé S. Fortin

3 Questions 5 Answers 0 Followers

Questions related from Chloé S. Fortin

Calculating Delta delta Cts for analysis qPCR results ?

Hi there, I want to use ddCt to analyze my qPCR results, however I am not exactly sure on how to proceed and when to average the replicates. Also, can we use ddCt when having more than 1 factor...

01 January 2020 1,377 6 View

Why do my KGN granulosa cells are shrinking when treated with PMA ?

Hello everyone I need help, I am working with KGN granulosa cell line (DMEM/F12 media, 10% FBS, 1% Pen/Strep) I am treating the cells with PMA at 100nM concentration to activate PKC, but...

04 April 2019 5,767 4 View

Calculating Delta delta Cts for analysis qPCR results ?

Hi there, I want to use ddCt to analyze my qPCR results, however I am not exactly sure on how to proceed and when to average the replicates. Also, can we use ddCt when having more than 1 factor...

01 January 1970 2,604 3 View