Chloe Doyle

2 Questions 1 Answers 0 Followers

Questions related from Chloe Doyle

Protocol for activating PBMCs using PHA/IL-2?

Hi there, just looking for a simple protocol stimulating 1 x 10^6 PBMCs/ mL in RMPI + 10%FBS using PHA and IL-2? I am looking for CD25, NKp44, c-kit expression and a few other markers for flow...

09 December 2020 2,344 1 View

Red Cell Lysis Buffer killing intestinal lymphocytes?! Help!

Hi there! I’m having trouble performing red cell lysis buffers. As well as killing red blood cells the buffer also seems to be killing the lymphocytes (Isolated from human intestinal tissue). My...

01 January 1970 770 3 View