Chitrasak Kullapanich

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Questions related from Chitrasak Kullapanich

What are possible reasons that my sequencing primers did not bind to the clusters in Illumina Miseq600 sequencing platform?

I ran an Illumina Miseq600 sequencing and it failed - a cluster density is only 79 k/mm2 with 0% passing filter. From the thumbnail image at 5 cycles, we detected background clusters in a large...

26 August 2020 2,738 2 View

How many bands should be collected for Library Prep when amplifying fungal ITS2 region?

I'm doing amplification of ITS2 region for fungal sequencing on Miseq. At times, 2 or three bands appeared on gel electrophoresis (range between 400-600 bp). Should all the appeared bands in this...

22 August 2020 3,741 3 View