3 Questions 9 Answers 0 Followers
Questions related from Chitra Latka
I am trying to purify a protein whose pI is around 9 and I am using cation exchanger (Mono-S) with buffer of pH 7. But, my protein always comes out in unbound fraction. I tried using Mono Q as...
03 March 2016 6,705 11 View
I am trying to purify a fungal alkaline serine protease. I have cloned it in pet28smt3 but the protein remained insoluble. Then, I cloned it in pGex4t1 which has GST tag, my protease after...
03 March 2015 4,003 8 View
I am trying to express my protein in Pichia pastoris X-33. I have cloned my gene in NotI and XbaI sites in pPICZalpha and after linearizing with PmeI and electroporation I plate on YPDS + Zeocin...
01 January 2015 5,565 2 View
