Chiti Agarwal

3 Questions 5 Answers 0 Followers

Questions related from Chiti Agarwal

How many mismatches to select from guide RNA to look for Crispr off target sites?

I am using cas-offinder to find mismatches. I found 1000s of sequences that differ from my guide RNA with minimum 4 nucleotides. I was thinking to consider the guide RNA with max 3 bases...

28 February 2022 103 1 View

How many peaks (fragment analysis: Microsatellite markers) should i see for halpoid species?

I got my fragment analysis results of Microsatellite markers designed using haploid species. I am using a gene marker to analyze the peaks. I read that since it is haploid, i should see only one...

25 February 2022 1,017 9 View

Are these two SSR/ microsatellite markers polymorphic?

I ran an agarose gel with 8 fungal isolates to check the polymorphism. I am not sure if I can consider the marker with the missing band as polymorphic?

16 November 2021 8,952 6 View