Could anyone suggest the logical percentage of F4/80+ cells in SIMPLE 7 day culture of bone marrow (media- RPMI+ 10% FCS)?

I am trying to develop macrophages from BALB/c bone marrow with an isolated polysaccharide of my own interest. During experiment i observed 45- 50% F4/80+ cells by FACS in 7 day culture (total...

13 September 2012 1,529 1 View