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I'm using VEGF to induce tube-like formation (matrigel ) of HMEC1 cells, however, I found some weird results that when I add VEGF to the medium, the tubes grow even less compare to my WT cells....
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I wonder if anybody met this situation before.. I ran a gel electrophoresis at 180v for 50 mins and do transfer in 20v 300mA 90mins. after transfering my protein to PVDF membrane, the ladder on...
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