Chinyere Kemet

2 Questions 1 Answers 0 Followers

Questions related from Chinyere Kemet

Why does my protein of interest get pulldown during IP, but not its complex memebers?

Hello, I am trying to conduct an IP between two proteins (protein A- 97kd and protein B-37kD). For my experiment, I am using 0.5X RIPA buffer with the addition of protease and phosphatase...

09 April 2024 1,403 0 View

Fluorescence Polarization: Non zero numbers for calibration curve?

Hi! I am doing FP/FA to study competitive binding interactions. I am currently doing experiments to determine the optimal buffer for my protein. From the literature, I am trying HEPES and tris...

07 December 2020 8,681 2 View