Ching Low

2 Questions 11 Answers 0 Followers

Questions related from Ching Low

GXL enzyme extension temperature 68 degrees?

Hi, is it alright to use an extension temperature of 68 degrees with GXL enzyme for PCR products below 4kb? Takara (GXL's manufacturer) recommends an extension temperature of 72 degrees with GXL...

04 February 2023 743 12 View

SuperFi II DNA polymerase results - one with no band and one with smear?

Hi, I am using the same DNA template to run two 7kb PCR experiments with the same cycling conditions simultaneously. The only difference between the two experiments is the primer pair. One result...

03 December 2022 4,793 7 View